翻訳と辞書 Words near each other ・ Two-way security ・ Two-way simultaneous ・ Two-way street ・ Two-graph ・ Two-Gun Gussie ・ Two-Gun Kid ・ Two-Gun Man from Harlem ・ Two-Gun of the Tumbleweed ・ Two-handed ・ Two-handed manual alphabets ・ Two-Handed Trans-Atlantic Race ・ Two-hander ・ Two-Headed Monster ・ Two-Headed Poems ・ Two-Higgs-doublet model ・ Two-hybrid screening ・ Two-in, two-out ・ Two-Lane Blacktop ・ Two-Lane Blacktop (song) ・ Two-lane expressway ・ Two-leaved Solomon's seal ・ Two-legged tie ・ Two-letter country codes ・ Two-letter English words ・ Two-level defense ・ Two-level game theory ・ Two-level grammar ・ Two-level scheduling ・ Two-level utilitarianism ・ Two-line element set Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Two-hybrid screening ： ウィキペディア英語版 Two-hybrid screening Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively. The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription.〔〔 The Y2H is thus a protein-fragment complementation assay. == History == Pioneered by Stanley Fields and Ok-Kyu Song in 1989, the technique was originally designed to detect protein–protein interactions using the GAL4 transcriptional activator of the yeast ''Saccharomyces cerevisiae''. The GAL4 protein activated transcription of a protein involved in galactose utilization, which formed the basis of selection.〔 Abstract is free; full-text article is not.〕 Since then, the same principle has been adapted to describe many alternative methods, including some that detect protein–DNA interactions or DNA-DNA interactions, as well as methods that use ''Escherichia coli'' instead of yeast.〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Two-hybrid screening」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.